Effects of iron-EDTA on uroporphyrinogen oxidation by liver microsomes

FEBS Lett. 1989 Jul 3;250(2):349-52. doi: 10.1016/0014-5793(89)80753-7.

Abstract

Uroporphyrinogen oxidation by hepatic microsomes from chick embryos or mice pretreated with methylcholanthrene was increased by addition of iron-EDTA. This increase was partially prevented by catalase, mannitol, ketoconazole and piperonyl butoxide, whereas only ketoconazole and piperonyl butoxide inhibited the oxidation in the presence and absence of iron-EDTA. These data suggest that the oxidations of uroporphyrinogen in the presence and absence of added iron occur by different mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Catalase / metabolism
  • Chick Embryo
  • Edetic Acid / pharmacology*
  • Ferric Compounds / pharmacology*
  • Iron Chelating Agents / pharmacology*
  • Ketoconazole / pharmacology
  • Mice
  • Microsomes, Liver / drug effects*
  • Microsomes, Liver / enzymology
  • Microsomes, Liver / metabolism
  • Oxidation-Reduction
  • Piperonyl Butoxide / pharmacology
  • Polychlorinated Biphenyls / pharmacology
  • Porphyrinogens / metabolism*
  • Superoxide Dismutase / metabolism
  • Uroporphyrinogens / metabolism*

Substances

  • Ferric Compounds
  • Iron Chelating Agents
  • Porphyrinogens
  • Uroporphyrinogens
  • Edetic Acid
  • Polychlorinated Biphenyls
  • Catalase
  • Superoxide Dismutase
  • Fe(III)-EDTA
  • Piperonyl Butoxide
  • Ketoconazole
  • 3,4,3',4'-tetrachlorobiphenyl