Immunoexpression of transforming growth factor beta and interferon gamma in radicular and dentigerous cysts

J Endod. 2014 Sep;40(9):1293-7. doi: 10.1016/j.joen.2014.01.010. Epub 2014 Jul 18.

Abstract

Introduction: The aim of this study was to evaluate and compare the immunohistochemical expression of transforming growing factor beta (TGF-β) and interferon gamma (IFN-γ) between radicular cysts (RCs) and dentigerous cysts (DCs).

Methods: Twenty RCs and DCs were selected for analysis of the immunoexpression of TGF-β and IFN-γ in the epithelium and capsule.

Results: The cell reactivity of TGF-β and IFN-γ in the lining epithelium and capsule of RCs showed no significant differences when compared with DCs (P > .05). There was a tendency of a higher expression of TGF-β in the capsule of DCs.

Conclusions: Our results showed the presence of TGF-β and IFN-γ in RCs and DCs, supporting the hypothesis that both participate in the development of these lesions, where IFN-γ usually plays a role in bone resorption, which is counterbalanced by the osteoprotective activity performed by TGF-β.

Keywords: Dentigerous cyst; immunohistochemistry; interferon gamma; radicular cyst; transforming growth factors.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Adult
  • Cell Nucleus / immunology
  • Child
  • Cytoplasm / immunology
  • Dentigerous Cyst / immunology*
  • Epithelial Cells / immunology
  • Epithelium / immunology
  • Female
  • Fibroblasts / immunology
  • Humans
  • Immunohistochemistry
  • Interferon-gamma / analysis*
  • Lymphocytes / immunology
  • Macrophages / immunology
  • Male
  • Middle Aged
  • Neutrophils / immunology
  • Plasma Cells / immunology
  • Radicular Cyst / immunology*
  • Transforming Growth Factor beta / analysis*
  • Young Adult

Substances

  • Transforming Growth Factor beta
  • Interferon-gamma