Abstract
Experimental autoimmune encephalomyelitis (EAE) is an autoimmune disease of the central nervous system (CNS) often used as a model for the early inflammatory stages of multiple sclerosis and also as a model of organ-specific autoimmune disease.This protocol describes the induction of passive EAE in mice, either using T cells isolated from mice primed with myelin antigens, or through the use of naïve TCR transgenic T cells activated in vitro in the presence of myelin-derived antigens.
MeSH terms
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Adjuvants, Immunologic
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Adoptive Transfer
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Animals
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CD4-Positive T-Lymphocytes / immunology
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CD4-Positive T-Lymphocytes / metabolism
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CD4-Positive T-Lymphocytes / transplantation*
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Disease Models, Animal*
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Encephalomyelitis, Autoimmune, Experimental / genetics*
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Encephalomyelitis, Autoimmune, Experimental / immunology*
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Encephalomyelitis, Autoimmune, Experimental / pathology
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Gene Transfer Techniques
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Mycobacterium tuberculosis / immunology
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Myelin Sheath / immunology*
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Receptors, Antigen, T-Cell / genetics*
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Th1 Cells / metabolism
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Th1 Cells / transplantation
Substances
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Adjuvants, Immunologic
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Receptors, Antigen, T-Cell