Rapid sequencing of the Mycobacterium tuberculosis pncA gene for detection of pyrazinamide susceptibility

J Clin Microbiol. 2014 Nov;52(11):4056-7. doi: 10.1128/JCM.02438-14. Epub 2014 Aug 27.

Abstract

We developed a pyrazinamidase gene DNA-sequencing method to rapidly identify pyrazinamide resistance-causing mutations in GenoLyse-treated, smear-positive sputum specimens. The sensitivity and specificity were 90.9 and 100%, respectively, compared to those of MGIT drug susceptibility testing, after the exclusion of synonymous mutations and nonsynonymous mutations previously associated with susceptibility to pyrazinamide.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Amidohydrolases / genetics*
  • Antitubercular Agents / pharmacology
  • Drug Resistance, Bacterial*
  • Genotype
  • Genotyping Techniques / methods*
  • Humans
  • Microbial Sensitivity Tests / methods
  • Mutation
  • Mycobacterium tuberculosis / drug effects
  • Mycobacterium tuberculosis / genetics*
  • Pyrazinamide / pharmacology
  • Sensitivity and Specificity
  • Sequence Analysis, DNA / methods*
  • Time Factors

Substances

  • Antitubercular Agents
  • Pyrazinamide
  • Amidohydrolases
  • PncA protein, Mycobacterium tuberculosis