Acute extravascular hemolytic transfusion reaction due to anti-Kpa antibody missed by electronic crossmatch

Transfus Apher Sci. 2014 Oct;51(2):168-71. doi: 10.1016/j.transci.2014.08.011. Epub 2014 Aug 15.

Abstract

Background: Kpa antigen is a low incidence red blood cell antigen within the Kell system. Anti-Kpa alloantibody may be associated with acute and delayed hemolytic transfusion reactions.

Case study: We report a case of a clinically significant acute extravascular hemolytic transfusion reaction mediated by previously unrecognized (and undetected) anti-Kpa alloantibody. This reaction occurred in a patient who met all criteria for electronic crossmatch, resulting in the transfusion of an incompatible red cell unit.

Results: Post-transfusion investigation showed the transfused red cell unit was crossmatch compatible at the immediate spin phase but was 3 + incompatible at the antiglobulin phase. No evidence of intravascular hemolysis was observed upon visual comparison of the pre- and post-transfusion peripheral blood plasma. Further testing showed the presence of anti-Kpa antibody. The clinical course of the patient included acute febrile and systemic reaction.

Conclusion: Acute extravascular hemolytic transfusion reaction may occur due to undetected anti-Kpa alloantibody. Various strategies for crossmatching are discussed in the context of antibodies to low incidence antigens.

Keywords: Acute extravascular hemolysis; Anti-Kpa antibody; Electronic crossmatch.

Publication types

  • Case Reports

MeSH terms

  • Blood Group Incompatibility / blood*
  • Blood Group Incompatibility / etiology*
  • Blood Grouping and Crossmatching*
  • Erythrocyte Transfusion / adverse effects*
  • Female
  • Hemolysis*
  • Humans
  • Isoantibodies / blood*
  • Medication Errors*
  • Membrane Glycoproteins / blood*
  • Metalloendopeptidases / blood*
  • Middle Aged

Substances

  • Isoantibodies
  • Membrane Glycoproteins
  • KEL protein, human
  • Metalloendopeptidases