Xpert(®) MTB/RIF assay for tuberculosis diagnosis: evaluation in an Indian setting

V P Myneedu; D Behera; A K Verma; M Bhalla; N Singh; J Arora; R Singhal; M Mathur; P Lal; R Sarin

doi:10.5588/ijtld.13.0328

Xpert(®) MTB/RIF assay for tuberculosis diagnosis: evaluation in an Indian setting

Int J Tuberc Lung Dis. 2014 Aug;18(8):958-60. doi: 10.5588/ijtld.13.0328.

Authors

V P Myneedu¹, D Behera¹, A K Verma¹, M Bhalla¹, N Singh¹, J Arora¹, R Singhal¹, M Mathur¹, P Lal¹, R Sarin¹

Affiliation

¹ Department of Microbiology, Lala Ram Sarup Institute of Tuberculosis and Respiratory Diseases, New Delhi, India.

PMID: 25199011
DOI: 10.5588/ijtld.13.0328

Abstract

The present study was conducted to evaluate the performance of the Xpert(®) MTB/RIF assay and compare Xpert results with solid and MGIT 960 liquid culture system. A total of 134 patients who had failed the Category I or II regimen were recruited for evaluation. Xpert correctly identified all Mycobacterium tuberculosis isolates. The sensitivity and specificity of the Xpert assay for the detection of rifampicin resistance was respectively 98.2% and 97.0% when compared with MGIT 960 results.

Publication types

Comparative Study

MeSH terms

Antibiotics, Antitubercular / pharmacology*
Bacteriological Techniques / methods
Drug Resistance, Bacterial
Humans
India
Microbial Sensitivity Tests
Mycobacterium tuberculosis / drug effects*
Mycobacterium tuberculosis / isolation & purification
Rifampin / pharmacology*
Sensitivity and Specificity
Tuberculosis / diagnosis*
Tuberculosis / drug therapy
Tuberculosis / microbiology

Substances

Antibiotics, Antitubercular
Rifampin