Isolation and characterization of microsatellite markers for Canavalia cathartica and C. lineata (Fabaceae)

Asuka Yamashiro; Tadashi Yamashiro; Yoichi Tateishi

doi:10.3732/apps.1200111

Isolation and characterization of microsatellite markers for Canavalia cathartica and C. lineata (Fabaceae)

Appl Plant Sci. 2013 Jan 2;1(1):apps.1200111. doi: 10.3732/apps.1200111. eCollection 2013 Jan.

Authors

Asuka Yamashiro¹, Tadashi Yamashiro², Yoichi Tateishi³

Affiliations

¹ Graduate School of Advanced Technology and Science, University of Tokushima, 2-1 Minami-josanjima, Tokushima 770-8506, Japan.
² Environmental Symbiosis Studies, Graduate School of Integrated Arts and Sciences, University of Tokushima, 1-1 Minami-josanjima, Tokushima 770-8502, Japan.
³ Department of Science Education, Faculty of Education, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan.

Abstract

Premise of the study: Microsatellite markers were developed for the sea-dispersed legume Canavalia cathartica to investigate its genetic diversity and population structure. We also applied these microsatellite markers to the closely related species C. lineata. •

Methods and results: Ten primer sets were developed for C. cathartica, and all of the primers were amplified in C. lineata. The number of alleles per locus ranged from two to 13 and one to 10 for C. cathartica and C. lineata, respectively. The expected heterozygosity ranged from 0.375 to 0.870 and from 0.071 to 0.877 for C. cathartica and C. lineata, respectively. •

Conclusions: The 10 microsatellite markers developed in this study can be used to analyze the population genetic structure of C. cathartica and other related taxa.

Keywords: Canavalia cathartica; Canavalia lineata; Fabaceae; microsatellite; polymorphism.