Cysteine proteinase genes in Indian strain of Haemonchus contortus

Mol Biochem Parasitol. 2014 Sep;196(2):117-21. doi: 10.1016/j.molbiopara.2014.09.003. Epub 2014 Sep 17.

Abstract

The genes of microvillar cysteine proteinases such as hmcp1, hmcp6 and gcp7 of phenotypically distinct morphotypes of female H. contortus having knobbed and linguiform vulvar flaps were cloned and characterized. Complementary DNA (cDNA) was synthesized from total RNA of both the morphotypes. The hmcp1, hmcp6 and gcp7 genes were amplified from cDNA. The amplicons were cloned in pDRIVE T/A cloning vector and sequenced by Sanger's dideoxy method. The nucleotide and deduced amino acid sequences were compared with the USA/UK strain H. contortus cysteine proteinase sequences. The cysteine proteinase genes of Indian strain show considerable variations with USA/UK strains. Our results revealed that Indian strain of H. contortus expresses both UK strain (hmcp1 and 6) and USA strain (gcp7) cysteine proteinases.

Keywords: Cysteine proteinase; Haemonchus contortus; Knobbed; Linguiform; gcp7; hmcp1; hmcp6.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Cysteine Proteases / chemistry
  • Cysteine Proteases / genetics*
  • Cysteine Proteases / metabolism
  • Female
  • Haemonchiasis / parasitology
  • Haemonchiasis / veterinary*
  • Haemonchus / chemistry
  • Haemonchus / classification
  • Haemonchus / enzymology*
  • Haemonchus / genetics
  • Helminth Proteins / chemistry
  • Helminth Proteins / genetics*
  • Helminth Proteins / metabolism
  • India
  • Male
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Alignment
  • Sheep
  • Sheep Diseases / parasitology*

Substances

  • Helminth Proteins
  • Cysteine Proteases