Directed Evolution of a Panel of Orthogonal T7 RNA Polymerase Variants for in Vivo or in Vitro Synthetic Circuitry

Adam J Meyer; Jared W Ellefson; Andrew D Ellington

doi:10.1021/sb500299c

Directed Evolution of a Panel of Orthogonal T7 RNA Polymerase Variants for in Vivo or in Vitro Synthetic Circuitry

ACS Synth Biol. 2015 Oct 16;4(10):1070-6. doi: 10.1021/sb500299c. Epub 2014 Oct 7.

Authors

Adam J Meyer¹, Jared W Ellefson¹, Andrew D Ellington¹

Affiliation

¹ Institute for Cellular and Molecular Biology, ‡Department of Chemistry and Biochemistry, and §Center for Systems & Synthetic Biology, University of Texas at Austin , Austin, Texas 78712, United States.

PMID: 25279711
DOI: 10.1021/sb500299c

Abstract

T7 RNA polymerase is the foundation of synthetic biological circuitry both in vivo and in vitro due to its robust and specific control of transcription from its cognate promoter. Here we present the directed evolution of a panel of orthogonal T7 RNA polymerase:promoter pairs that each specifically recognizes a synthetic promoter. These newly described pairs can be used to independently control up to six circuits in parallel.

Keywords: directed evolution; genetic circuits; transcription.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA-Directed RNA Polymerases / genetics*
Promoter Regions, Genetic / genetics*
Viral Proteins / genetics*

Substances

Viral Proteins
bacteriophage T7 RNA polymerase
DNA-Directed RNA Polymerases