A real-time PCR assay for the diagnosis of gastrointestinal nematode infections of small ruminants

Florian Roeber; Aaron R Jex; Robin B Gasser

doi:10.1007/978-1-4939-2004-4_10

A real-time PCR assay for the diagnosis of gastrointestinal nematode infections of small ruminants

Methods Mol Biol. 2015:1247:145-52. doi: 10.1007/978-1-4939-2004-4_10.

Authors

Florian Roeber¹, Aaron R Jex, Robin B Gasser

Affiliation

¹ Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia.

PMID: 25399093
DOI: 10.1007/978-1-4939-2004-4_10

Abstract

The diagnosis of gastrointestinal nematode infections in small ruminants is central to studying the biology and epidemiology of these parasites and underpins their control. Traditional methods of diagnosis are inaccurate, time-consuming and laborious. Here, we describe a step-by-step protocol for the molecular-based diagnosis of infections by real-time PCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animal Diseases / diagnosis*
Animal Diseases / parasitology*
Animals
Gastrointestinal Diseases / veterinary*
Nematoda / genetics*
Nematode Infections / veterinary*
Real-Time Polymerase Chain Reaction / methods*
Ruminants*