Anaerobic mineralization of 2,4,6-tribromophenol to CO2 by a synthetic microbial community comprising Clostridium, Dehalobacter, and Desulfatiglans

Bioresour Technol. 2015 Jan:176:225-32. doi: 10.1016/j.biortech.2014.10.097. Epub 2014 Oct 25.

Abstract

Anaerobic mineralization of 2,4,6-tribromophenol (2,4,6-TBP) was achieved by a synthetic anaerobe community comprising a highly enriched culture of Dehalobacter sp. phylotype FTH1 acting as a reductive debrominator; Clostridium sp. strain Ma13 acting as a hydrogen supplier via glucose fermentation; and a novel 4-chlorophenol-degrading anaerobe, Desulfatiglans parachlorophenolica strain DS. 2,4,6-TBP was debrominated to phenol by the combined action of Ma13 and FTH1, then mineralized into CO2 by sequential introduction of DS, confirmed using [ring-(14)C(U)] phenol. The optimum concentrations of glucose, SO4(2-), and inoculum densities were 0.5 or 2.5mM, 1.0 or 2.5mM, and the densities equivalent to 10(4)copiesmL(-1) of the 16S rRNA genes, respectively. This resulted in the complete mineralization of 23μM 2,4,6-TBP within 35days (0.58μmolL(-1)d(-1)). Thus, using a synthetic microbial community of isolates or highly enriched cultures would be an efficient, optimizable, low-cost strategy for anaerobic bioremediation of halogenated aromatics.

Keywords: 2,4,6-Tribromophenol; Anaerobic mineralization; Synthetic anaerobe community; The optimized conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaerobiosis
  • Batch Cell Culture Techniques
  • Biodegradation, Environmental
  • Carbon Dioxide / metabolism*
  • Clostridium / metabolism*
  • Deltaproteobacteria / metabolism*
  • Environmental Restoration and Remediation / methods
  • Kinetics
  • Peptococcaceae / metabolism*
  • Phenols / metabolism*

Substances

  • Phenols
  • Carbon Dioxide
  • 2,4,6-tribromophenol