Fast analysis of polyphenols in royal jelly products using automated TurboFlow™-liquid chromatography-Orbitrap high resolution mass spectrometry

Noelia López-Gutiérrez; María Del Mar Aguilera-Luiz; Roberto Romero-González; José Luis Martínez Vidal; Antonia Garrido Frenich

doi:10.1016/j.jchromb.2014.09.038

Fast analysis of polyphenols in royal jelly products using automated TurboFlow™-liquid chromatography-Orbitrap high resolution mass spectrometry

J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Dec 15:973C:17-28. doi: 10.1016/j.jchromb.2014.09.038. Epub 2014 Oct 21.

Authors

Noelia López-Gutiérrez¹, María Del Mar Aguilera-Luiz¹, Roberto Romero-González¹, José Luis Martínez Vidal¹, Antonia Garrido Frenich²

Affiliations

¹ Group "Analytical Chemistry of Contaminants", Department of Chemistry and Physics (Analytical Chemistry Area), Research Centre for Agricultural and Food Biotechnology (BITAL), University of Almería, Agrifood Campus of International Excellence, ceiA3, Carretera de Sacramento s/n, E-04120 Almería, Spain.
² Group "Analytical Chemistry of Contaminants", Department of Chemistry and Physics (Analytical Chemistry Area), Research Centre for Agricultural and Food Biotechnology (BITAL), University of Almería, Agrifood Campus of International Excellence, ceiA3, Carretera de Sacramento s/n, E-04120 Almería, Spain. Electronic address: agarrido@ual.es.

PMID: 25464090
DOI: 10.1016/j.jchromb.2014.09.038

Abstract

This study describes the development of a novel, simple and fast analytical method for the detection and quantification of polyphenols in royal jelly products, using an in-house database containing more than 50 compounds. The extraction method consisted of sample dilution, followed by a fast on-line system composed of turbulent flow chromatography (TurboFlow™) coupled to liquid chromatography (LC)-Exactive-Orbitrap analyzer. The total run time was 18min, including automated extraction, analytical chromatography and re-equilibration. The method was validated obtaining limits of quantification (LOQ) ranging from 10 to 150μg/kg. The linearity range was up to 2000μg/L and determination coefficients (R²) were higher than 0.994. Adequate recoveries were obtained at three concentration levels (500, 1000 and 2000μg/kg). This method was applied to the analysis of nine samples and the concentration of polyphenols ranged from 14 (apigenin) to 18,936μg/kg (ferulic acid).

Keywords: Polyphenol; Royal jelly; TurboFlow; UHPLC–Orbitrap.