Neisseria meningitidis porA, fetA and fHbp gene distribution in Western Australia 2000 to 2011

BMC Infect Dis. 2014 Dec 12:14:686. doi: 10.1186/s12879-014-0686-x.

Abstract

Background: PorA, fetA and fHbp are three antigen encoding genes useful for meningococcal typing and FHbp is an important component of meningococcal B vaccines.

Methods: We performed sequence analysis of meningococcal porA, fetA and fHbp genes on 128 isolates from Western Australia, relating results to age, gender, race and geographic region.

Results: We found predominantly PorA subtypes P1.22,14-16 (n = 23) and P1.7-2,4 (n = 19); FetA subtypes F1-5 (n = 41), F3-6 (n = 11), F5-1 (n = 10), F5-2 (n = 9), F5-5 (n = 8), F3-3 (n = 8); and FHbp variant groups 1 (n = 65) and 2 (n = 44). PorA P1.22,14-16 and FHbp variant group 2 were associated with younger age and aboriginality.

Conclusions: FHbp modular groups of the bivalent recombinant FHbp vaccine and the multicomponent 4CMenB vaccine make up 8.3% and 47.7% respectively of the examined serogroup B isolates from 2000-2011, however to estimate vaccine efficacy requires an account of all vaccine antigens and their levels of expression.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Antigens, Bacterial / genetics*
  • Bacterial Outer Membrane Proteins / genetics*
  • Bacterial Proteins / genetics*
  • Child
  • Child, Preschool
  • Female
  • Genotype
  • Humans
  • Infant
  • Male
  • Meningococcal Infections / microbiology*
  • Meningococcal Infections / prevention & control
  • Meningococcal Vaccines
  • Middle Aged
  • Neisseria meningitidis / genetics*
  • Neisseria meningitidis / immunology
  • Porins / genetics*
  • Sequence Analysis, DNA
  • Vaccination
  • Western Australia
  • Young Adult

Substances

  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • FrpB protein, bacteria
  • Meningococcal Vaccines
  • Porins
  • factor H-binding protein, Neisseria meningitidis
  • porin protein, Neisseria