Abstract
Induced pluripotent stem cells from nonhuman primates (NHPs) have unique roles in cell biology and regenerative medicine. Because of the relatedness of NHPs to humans, NHP iPS cells can serve as a source of differentiated derivatives that can be used to address important questions in the comparative biology of primates. Additionally, when used as a source of cells for regenerative medicine, NHP iPS cells serve an invaluable role in translational experiments in cell therapy. Reprogramming of NHP somatic cells requires the same conditions as previously established for human cells. However, throughout the process, a variety of modifications to the human cell protocols must be made to accommodate significant species differences.
Keywords:
Induced pluripotent stem cells; Marmoset; Nonhuman primates; Reprogramming; Retroviruses; Teratoma.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Callithrix
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Cell Culture Techniques / methods
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Cells, Cultured
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Cellular Reprogramming
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Cellular Reprogramming Techniques / methods
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Fibroblasts / cytology
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Genes, myc
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Genetic Vectors / genetics
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Humans
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Induced Pluripotent Stem Cells / cytology*
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Induced Pluripotent Stem Cells / transplantation
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Kruppel-Like Factor 4
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Kruppel-Like Transcription Factors / genetics
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Kruppel-Like Transcription Factors / physiology
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Mice
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Mice, Knockout
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Octamer Transcription Factor-3 / genetics
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Octamer Transcription Factor-3 / physiology
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Primates*
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Proto-Oncogene Proteins c-myb / physiology
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Retroviridae / genetics
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SOXB1 Transcription Factors / genetics
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SOXB1 Transcription Factors / physiology
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Species Specificity
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Teratoma / pathology
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Transgenes*
Substances
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Kruppel-Like Factor 4
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Kruppel-Like Transcription Factors
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Octamer Transcription Factor-3
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Proto-Oncogene Proteins c-myb
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SOXB1 Transcription Factors