Complete Genome Sequence of Steroid-Transforming Nocardioides simplex VKM Ac-2033D

Victoriya Y Shtratnikova; Mikhail I Schelkunov; Yury A Pekov; Victoria V Fokina; Mariya D Logacheva; Sergey L Sokolov; Eugeny Y Bragin; Vasily V Ashapkin; Marina V Donova

doi:10.1128/genomeA.01406-14

Complete Genome Sequence of Steroid-Transforming Nocardioides simplex VKM Ac-2033D

Genome Announc. 2015 Jan 8;3(1):e01406-14. doi: 10.1128/genomeA.01406-14.

Authors

Victoriya Y Shtratnikova¹, Mikhail I Schelkunov¹, Yury A Pekov¹, Victoria V Fokina², Mariya D Logacheva, Sergey L Sokolov², Eugeny Y Bragin², Vasily V Ashapkin³, Marina V Donova⁴

Affiliations

¹ Department of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia.
² G.K. Skryabin Institute of Biochemistry & Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia.
³ A.N. Belozersky Department of Bioengineering and Bioinformatics, M.V. Lomonosov Moscow State University, Moscow, Russia.
⁴ G.K. Skryabin Institute of Biochemistry & Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia donova@ibpm.pushchino.ru.

Abstract

Nocardioides simplex VKM Ac-2033D is an effective microbial catalyst for 3-ketosteroid 1(2)-dehydrogenation, and it is capable of effective reduction of carbonyl groups at C-17 and C-20, hydrolysis of acetylated steroids, and utilization of natural sterols. Here, the complete genome sequence is reported. An array of genes related to steroid metabolic pathways have been identified.