Aldosterone induces a rapid increase in the rate of Na,K-ATPase gene transcription in cultured kidney cells

Mol Endocrinol. 1989 Sep;3(9):1369-76. doi: 10.1210/mend-3-9-1369.

Abstract

Aldosterone (300 nM) induces a 4-fold increase over a 6-hr stimulation in A6 kidney cells from Xenopus laevis in the abundance of mRNA beta 1 and a 2-fold in that of mRNA alpha 1 coding for each Na,K-ATPase subunit, which is in agreement with a previous report. After a 3-hr stimulation already, aldosterone elicited a significant increase of mRNA beta 1 (2.51-fold +/- 0.47, n = 3, P less than 0.05) and a nonsignificant increase of mRNA alpha 1 (1.26-fold +/- 0.30, n = 3, NS). Increasing doses of cycloheximide up to 3 micrograms ml-1 led to 90% inhibition of protein synthesis, but failed to block the differential effect of aldosterone on mRNA abundance over a 6-h incubation period. The rate of transcription was measured by a nuclear run-on assay. The basal rate of mRNA alpha 1 transcription exceeded that of mRNA beta 1 by 2.8-fold. Aldosterone (300 nM) stimulated the transcription of the two subunit genes. Fifteen minutes after aldosterone addition there was a significant and parallel increase in the rate of transcription of the alpha 1 subunit (1.98-fold +/- 0.20, n = 3, P less than 0.02) and that of the beta 1 subunit (2.13 +/- 0.32, n = 3, P less than 0.04). After a 45-min stimulation period the transcription rate of the alpha 1 subunit remained at the level observed at 15 min (1.84-fold +/- 0.14, n = 4, P less than 0.01), while the transcription rate of the beta 1 subunit increased further (2.89-fold +/- 0.38, n = 4, P less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldosterone / pharmacology*
  • Animals
  • Blotting, Northern
  • Cells, Cultured
  • Cycloheximide / pharmacokinetics
  • Gene Expression / drug effects
  • In Vitro Techniques
  • Kidney / metabolism*
  • RNA, Messenger / biosynthesis
  • Sodium-Potassium-Exchanging ATPase / biosynthesis*
  • Sodium-Potassium-Exchanging ATPase / genetics
  • Transcription, Genetic / drug effects*
  • Xenopus laevis

Substances

  • RNA, Messenger
  • Aldosterone
  • Cycloheximide
  • Sodium-Potassium-Exchanging ATPase