Glycosylation of mouse DPP4 plays a role in inhibiting Middle East respiratory syndrome coronavirus infection

J Virol. 2015 Apr;89(8):4696-9. doi: 10.1128/JVI.03445-14. Epub 2015 Feb 4.

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) utilizes dipeptidyl peptidase 4 (DPP4) as an entry receptor. Mouse DPP4 (mDPP4) does not support MERS-CoV entry; however, changes at positions 288 and 330 can confer permissivity. Position 330 changes the charge and glycosylation state of mDPP4. We show that glycosylation is a major factor impacting DPP4 receptor function. These results provide insight into DPP4 species-specific differences impacting MERS-CoV host range and may inform MERS-CoV mouse model development.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Coronavirus Infections / prevention & control*
  • Dipeptidyl Peptidase 4 / chemistry
  • Dipeptidyl Peptidase 4 / genetics
  • Dipeptidyl Peptidase 4 / metabolism*
  • Fluorescent Antibody Technique
  • Glycosylation
  • Mice
  • Middle East Respiratory Syndrome Coronavirus / metabolism*
  • Models, Molecular*
  • Molecular Sequence Data
  • Species Specificity
  • Virus Internalization*

Substances

  • Dipeptidyl Peptidase 4
  • Dpp4 protein, mouse

Associated data

  • PDB/4L72