Abstract
A novel method based on the conjunction of aldehydes from oxidized glycopeptides to aniline groups on magnetic nanoparticles via nonreductive amination is reported for the highly selective enrichment of N-glycopeptides. For the first time, a nonreductive amination reaction has been introduced into N-glycoproteome extraction, and correspondingly a new type of aniline-functionalized nanoparticle has been designed and synthesized.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Aldehydes / chemistry
-
Amination
-
Aniline Compounds / chemistry
-
Animals
-
Asialoglycoproteins / isolation & purification*
-
Blood Proteins / chemistry*
-
Cattle
-
Ferrosoferric Oxide / chemistry*
-
Fetuins / isolation & purification*
-
Glycopeptides / isolation & purification*
-
Humans
-
Magnetite Nanoparticles / chemistry*
-
Proteome / isolation & purification*
-
Silicon Dioxide / chemistry*
-
Solid Phase Microextraction / methods
Substances
-
Aldehydes
-
Aniline Compounds
-
Asialoglycoproteins
-
Blood Proteins
-
Fetuins
-
Glycopeptides
-
Magnetite Nanoparticles
-
Proteome
-
asialofetuin
-
Silicon Dioxide
-
aniline
-
Ferrosoferric Oxide