Comparison of the Vidas C. difficile GDH Automated Enzyme-Linked Fluorescence Immunoassay (ELFA) with Another Commercial Enzyme Immunoassay (EIA) (Quik Chek-60), Two Selective Media, and a PCR Assay for gluD for Detection of Clostridium difficile in Fecal Samples

J Clin Microbiol. 2015 Jun;53(6):1931-4. doi: 10.1128/JCM.00649-15. Epub 2015 Mar 18.

Abstract

Prevention and management of Clostridium difficile infection (CDI) can be improved by rapid and reliable diagnostics. The Vidas C. difficile glutamate dehydrogenase assay had performance comparable to that of the Quik Chek-60 assay (overall agreement, 95%) and a sensitivity of >93%; thus, it is suitable as the first test in two-stage algorithms for a CDI diagnosis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / genetics*
  • Adolescent
  • Adult
  • Bacterial Proteins / genetics*
  • Bacterial Typing Techniques / methods*
  • Child
  • Child, Preschool
  • Clostridioides difficile / genetics
  • Clostridioides difficile / isolation & purification*
  • Culture Media
  • Enterocolitis, Pseudomembranous / diagnosis
  • Enterocolitis, Pseudomembranous / microbiology
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Feces / microbiology*
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Middle Aged
  • Polymerase Chain Reaction / methods*
  • Prospective Studies
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Young Adult

Substances

  • ATP-Binding Cassette Transporters
  • Bacterial Proteins
  • Culture Media
  • GluD protein, Corynebacterium glutamicum