A DNA logic gate based on strand displacement reaction and rolling circle amplification, responding to multiple low-abundance DNA fragment input signals, and its application in detecting miRNAs

Yuqi Chen; Yanyan Song; Fan Wu; Wenting Liu; Boshi Fu; Bingkun Feng; Xiang Zhou

doi:10.1039/c5cc01389e

A DNA logic gate based on strand displacement reaction and rolling circle amplification, responding to multiple low-abundance DNA fragment input signals, and its application in detecting miRNAs

Chem Commun (Camb). 2015 Apr 25;51(32):6980-3. doi: 10.1039/c5cc01389e. Epub 2015 Mar 23.

Authors

Yuqi Chen¹, Yanyan Song, Fan Wu, Wenting Liu, Boshi Fu, Bingkun Feng, Xiang Zhou

Affiliation

¹ College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Wuhan University, Wuhan, Hubei 430072, P. R. China. xzhou@whu.edu.cn.

PMID: 25797704
DOI: 10.1039/c5cc01389e

Abstract

A conveniently amplified DNA AND logic gate platform was designed for the highly sensitive detection of low-abundance DNA fragment inputs based on strand displacement reaction and rolling circle amplification strategy. Compared with others, this system can detect miRNAs in biological samples. The success of this strategy demonstrates the potential of DNA logic gates in disease diagnosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Computers, Molecular*
DNA / chemistry*
DNA / genetics
HeLa Cells
Humans
Limit of Detection
Logic*
MicroRNAs / analysis*
MicroRNAs / genetics*
Nucleic Acid Amplification Techniques

Substances

MicroRNAs
DNA