Amphipathic DNA origami nanoparticles to scaffold and deform lipid membrane vesicles

Angew Chem Int Ed Engl. 2015 May 26;54(22):6501-5. doi: 10.1002/anie.201501173. Epub 2015 Apr 16.

Abstract

We report a synthetic biology-inspired approach for the engineering of amphipathic DNA origami structures as membrane-scaffolding tools. The structures have a flat membrane-binding interface decorated with cholesterol-derived anchors. Sticky oligonucleotide overhangs on their side facets enable lateral interactions leading to the formation of ordered arrays on the membrane. Such a tight and regular arrangement makes our DNA origami capable of deforming free-standing lipid membranes, mimicking the biological activity of coat-forming proteins, for example, from the I-/F-BAR family.

Keywords: diffusion; lipid membranes; membrane deformation; oligomerization; origami DNA structures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cholesterol / chemistry
  • DNA / chemistry*
  • Diffusion
  • Fluorescent Dyes / chemistry
  • Membrane Lipids / chemistry*
  • Microscopy, Fluorescence
  • Nanoparticles / chemistry*
  • Nanoparticles / ultrastructure
  • Oligonucleotides / chemistry
  • Unilamellar Liposomes / chemistry*

Substances

  • Fluorescent Dyes
  • Membrane Lipids
  • Oligonucleotides
  • Unilamellar Liposomes
  • DNA
  • Cholesterol