In-vitro model for studying methanogens in human gut microbiota

William Tottey; Nadia Gaci; Guillaume Borrel; Monique Alric; Paul W O'Toole; Jean-François Brugère

doi:10.1016/j.anaerobe.2015.04.009

In-vitro model for studying methanogens in human gut microbiota

Anaerobe. 2015 Aug:34:50-2. doi: 10.1016/j.anaerobe.2015.04.009. Epub 2015 Apr 14.

Authors

William Tottey¹, Nadia Gaci¹, Guillaume Borrel², Monique Alric¹, Paul W O'Toole², Jean-François Brugère³

Affiliations

¹ EA-4678 CIDAM, Clermont Université, Université d'Auvergne, Clermont-Ferrand, France.
² School of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Ireland.
³ EA-4678 CIDAM, Clermont Université, Université d'Auvergne, Clermont-Ferrand, France. Electronic address: jf.brugere@udamail.fr.

PMID: 25887578
DOI: 10.1016/j.anaerobe.2015.04.009

Abstract

Reported failures with gnotobiotic animal models led us to establish an in-vitro model of reciprocal conversion of methanogenic and non methanogenic microbiota from human fecal samples. Consequences on gas and microbiota compositions are reported. This should facilitate the study of the controversial role of gut methanogens in human health.

Keywords: Colon; ECSIM; Gut microbiota; Human gut archaea; In vitro systems; Methanobrevibacter smithii.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gastrointestinal Microbiome*
Humans
Methane / metabolism*
Microbiological Techniques / methods*
Microbiota*
Models, Theoretical*

Substances

Methane