Phloem restriction of viroids in three citrus hosts is overcome by grafting with Etrog citron: potential involvement of a translocatable factor

J Gen Virol. 2015 Aug;96(8):2405-2410. doi: 10.1099/vir.0.000154. Epub 2015 Apr 17.

Abstract

Viroid systemic spread involves cell-to-cell movement from initially infected cells via plasmodesmata, long-distance movement within the phloem and again cell-to-cell movement to invade distal tissues including the mesophyll. Citrus exocortis viroid (CEVd), hop stunt viroid, citrus bent leaf viroid, citrus dwarfing viroid, citrus bark cracking viroid and citrus viroid V remained phloem restricted when singly infecting Citrus karna, Citrus aurantium and Poncirus trifoliata, but not Etrog citron, where they were additionally detected in mesophyll protoplasts. However, when CEVd-infected C. karna was side-grafted with Etrog citron--with the resulting plants being composed of a C. karna stock and an Etrog citron branch--the viroid was detected in mesophyll protoplasts of the former, thus indicating that the ability of Etrog citron to support viroid invasion of non-vascular tissues was transferred to the stock. Further results suggest that a translocatable factor from Etrog citron mediates this viroid trafficking.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breeding
  • Citrus / classification
  • Citrus / genetics
  • Citrus / metabolism
  • Citrus / virology*
  • Host Specificity*
  • Phloem / virology*
  • Plant Diseases / genetics
  • Plant Diseases / virology*
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Viroids / genetics
  • Viroids / isolation & purification
  • Viroids / physiology*

Substances

  • Plant Proteins