High Molecular Weight Typing with MALDI-TOF MS - A Novel Method for Rapid Typing of Clostridium difficile

PLoS One. 2015 Apr 29;10(4):e0122457. doi: 10.1371/journal.pone.0122457. eCollection 2015.

Abstract

Clostridium difficile strains were typed by a newly developed MALDI-TOF method, high molecular weight typing, and compared to PCR ribotyping. Among 500 isolates representing 59 PCR ribotypes a total of 35 high molecular weight types could be resolved. Although less discriminatory than PCR ribotyping, the method is extremely fast and simple, and supports for cost-effective screening of isolates during outbreak situations.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques / economics
  • Bacterial Typing Techniques / methods*
  • Clostridioides difficile / chemistry*
  • Clostridioides difficile / genetics
  • Clostridioides difficile / isolation & purification
  • Clostridium Infections / diagnosis
  • Clostridium Infections / microbiology*
  • Humans
  • Molecular Weight
  • Polymerase Chain Reaction / methods
  • Ribotyping / economics
  • Ribotyping / methods
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / economics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Grants and funding

The study was supported by the Swedish Civil Contingencies Agency. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.