During three months of sampling, one thousand nine hundred and thirty five sand flies belonging to thirteen species of Phlebotomine sandflies were collected in suburban location of Bamako. Phlebotomus duboscqi, which is the common vector of cutaneous leishmaniasis in Mali, was found for the first time in Bamako mostly within human houses, which can confirm the possibility of a local transmission of Leishmania major. Sergentomyia freetownensis was found for the first time in Mali, which raises to 15 the number of sand flies species identified in Mali.