High-risk HPV detection and genotyping by APTIMA HPV using cervical samples

J Virol Methods. 2015 Sep 1:221:95-9. doi: 10.1016/j.jviromet.2015.04.030. Epub 2015 May 5.

Abstract

High-risk human papillomavirus (HPV) detection and genotyping is critical for cervical cancer screening. Testing of 967 cervical cytology specimens in PreservCyt preservative revealed similar positivity rates for HC2 (13.8%) and APTIMA HPV (AHPV) tests (13.5%, p=0.89) and high overall agreement (94.6%, κ=0.77). A trend towards higher HPV16 positivity rates by the Cobas HPV test (23.0%, 26/113) compared to the AHPV genotyping assay (19.5%, 22/113; p=0.125) was noted. No cross-contamination was detected with AHPV in a challenge experiment.

Keywords: HPV.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Cervix Uteri / virology*
  • Female
  • Genotyping Techniques / methods*
  • Humans
  • Middle Aged
  • Molecular Diagnostic Techniques / methods*
  • Papillomaviridae / classification*
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification*
  • Papillomavirus Infections / diagnosis*
  • Papillomavirus Infections / virology*
  • Virology / methods