Candida rugosa lipase immobilization on hydrophilic charged gold nanoparticles as promising biocatalysts: Activity and stability investigations

Iole Venditti; Cleofe Palocci; Laura Chronopoulou; Ilaria Fratoddi; Laura Fontana; Marco Diociaiuti; Maria Vittoria Russo

doi:10.1016/j.colsurfb.2015.04.046

Candida rugosa lipase immobilization on hydrophilic charged gold nanoparticles as promising biocatalysts: Activity and stability investigations

Colloids Surf B Biointerfaces. 2015 Jul 1:131:93-101. doi: 10.1016/j.colsurfb.2015.04.046. Epub 2015 Apr 27.

Authors

Iole Venditti¹, Cleofe Palocci², Laura Chronopoulou¹, Ilaria Fratoddi³, Laura Fontana¹, Marco Diociaiuti⁴, Maria Vittoria Russo⁵

Affiliations

¹ Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy.
² Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy. Electronic address: cleofe.palocci@uniroma1.it.
³ Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy; Center for Nanotechnology for Engineering (CNIS), Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy. Electronic address: ilaria.fratoddi@uniroma1.it.
⁴ Technology and Health Department, Istituto Superiore di Sanità, viale Regina Elena 299, 00161 Rome, Italy.
⁵ Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy; Center for Protection of Environment and Cultural Heritages (CIABC), Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy.

PMID: 25969418
DOI: 10.1016/j.colsurfb.2015.04.046

Abstract

In this work, a simple and versatile methodology to obtain two different bioconjugated systems has been developed by the immobilization of Candida rugosa lipase (CRL) on hydrophilic gold nanoparticles functionalized with 2-diethylaminoethanethiol hydrochloride (DEA) or with sodium 3-mercapto-1-propanesulfonate (3MPS), namely Au-DEA@CRL and Au-3MPS@CRL. Both spectroscopic and morphological properties of metal nanoparticles have been deeply investigated. The enzyme loading and lipolytic activity of AuNPs@CRL bioconjugates have been studied with respect to different surface functionalization and compared with the free enzyme. Some physical and chemical parameters had a strong effect on enzyme activity and stability, that were improved in the case of the Au-DEA@CRL bioconjugate, which showed a remarkable biocatalytic performance (95% of residual lipolytic activity compared with free CRL) and stability in experimental conditions concerning pH (range 5-8) and temperature (range 20-60°C), as often required for the industrial scale up of catalytic systems.

Keywords: Biocatalysis; Candida rugosa lipase; Gold nanoparticles; Lipase immobilization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biocatalysis
Candida / enzymology*
Enzyme Stability
Enzymes, Immobilized / chemistry
Enzymes, Immobilized / metabolism
Fungal Proteins / chemistry
Fungal Proteins / metabolism*
Gold / chemistry*
Hydrogen-Ion Concentration
Hydrophobic and Hydrophilic Interactions
Lipase / chemistry
Lipase / metabolism*
Lipolysis
Metal Nanoparticles / chemistry*
Metal Nanoparticles / ultrastructure
Microscopy, Atomic Force
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Molecular Structure
Sulfhydryl Compounds / chemistry
Temperature

Substances

3-mercapto-1-propanesulfonate
Enzymes, Immobilized
Fungal Proteins
Sulfhydryl Compounds
Gold
Lipase