Objective: To investigate the association between CCND1 polymorphisms and the DNA and chromosome damage in peripheral blood lymphocytes among workers exposed to 1,3-butadiene(BD).
Methods: 171 workers exposed to 1,3-butadiene and 55 controls without occupational BD exposure were investigated. Cytokinesis-block micronucleus(CBMN) detection and comet assay were used to evaluate the chromosomal and DNA damage levels in peripheral blood lymphocyte. And the genotypes of CCND1 were detected by PCR-RFLP. Personal information including occupational history, age, sex, smoking and drinking status was collected by the questionnaire.
Results: The rate of CBMN and OTM of lymphocyte in BD exposed workers[(7. 17 ± 5. 09)% and 4. 65(95% CI 3. 49 - 5. 98), respectively] were higher than those in controls[(3. 82 ± 2. 37)% and 2. 38 (95% CI 0. 82 - 3. 98), P < 0. 01. Multivariate analysis of covariance revealed that in BD workers. The subjects with CCND1 A870G AA allele have significantly higher risk for DNA damage than subjects with GG allele (P < 0. 05).
Conclusion: Under present BD exposure levels, both comet assay and cytokinesis-block micronucleus test could be sensitive group biomarkers to use to monitor the genotoxicity of BD occupational exposure. CCNDI A870G polymorphisms might influence the susceptibility of DNA damage in BD exposed workers.