Deep Sequencing of HIV-1 in Cerebrospinal Fluid

Abstract

Using deep sequencing, human immunodeficiency virus (HIV) resistance-associated mutations were detected as minority species in the cerebrospinal fluid (CSF) of 4 patients with higher HIV type 1 RNA load in CSF than in plasma, but not in 2 patients with higher plasma viral load. Deep sequencing could help our understanding of viral escape in the central nervous system.

Keywords: CNS penetration; deep sequencing; minority species; viral escape.