Population genetic analysis of insertion-deletion polymorphisms in a Brazilian population using the Investigator DIPplex kit

Teresinha de Jesus Brabo Ferreira Palha; Elzemar Martins Ribeiro Rodrigues; Giovanna Chaves Cavalcante; Andrea Marrero; Ilíada Rainha de Souza; Clineu Julien Seki Uehara; Carlos Henrique Ares Silveira da Motta; Daniela Koshikene; Dayse Aparecida da Silva; Elizeu Fagundes de Carvalho; Gustavo Chemale; Jorge M Freitas; Lídia Alexandre; Renato T F Paranaiba; Mirella Perruccio Soler; Sidney Santos

doi:10.1016/j.fsigen.2015.03.015

Population genetic analysis of insertion-deletion polymorphisms in a Brazilian population using the Investigator DIPplex kit

Forensic Sci Int Genet. 2015 Nov:19:10-14. doi: 10.1016/j.fsigen.2015.03.015. Epub 2015 Apr 24.

Authors

Teresinha de Jesus Brabo Ferreira Palha¹, Elzemar Martins Ribeiro Rodrigues¹, Giovanna Chaves Cavalcante², Andrea Marrero³, Ilíada Rainha de Souza³, Clineu Julien Seki Uehara⁴, Carlos Henrique Ares Silveira da Motta⁵, Daniela Koshikene⁶, Dayse Aparecida da Silva⁷, Elizeu Fagundes de Carvalho⁷, Gustavo Chemale⁸, Jorge M Freitas⁸, Lídia Alexandre⁸, Renato T F Paranaiba⁸, Mirella Perruccio Soler⁹, Sidney Santos¹⁰

Affiliations

¹ Laboratório de Genética Humana e Médica - Universidade Federal do Pará - UFPA, PA, Brazil; Centro de Pericias Científicas Renato Chaves, PA, Brazil.
² Laboratório de Genética Humana e Médica - Universidade Federal do Pará - UFPA, PA, Brazil.
³ Laboratório de Polimorfismos Genéticos, Universidade Federal de Santa Catarina, SC, Brazil.
⁴ Instituto Geral de Perícias de Santa Catarina, SC, Brazil.
⁵ Setor de Genética Molecular - Instituto de Medicina Social e de Criminologia de São Paulo, SP, Brazil.
⁶ Laboratório de Genética Forense - Polícia Civil do Amazonas, AM, Brazil.
⁷ Laboratório de Diagnósticos por DNA - Universidade do Estado do Rio de Janeiro, RJ, Brazil.
⁸ Laboratório de Genética Forense - Instituto Nacional de Criminalística - Diretoria Técnico-Científica - Polícia Federal - Ministério da Justiça - Brasília, DF, Brazil.
⁹ QIAGEN Biotecnologia, SP, Brazil.
¹⁰ Laboratório de Genética Humana e Médica - Universidade Federal do Pará - UFPA, PA, Brazil. Electronic address: sidneysantos@ufpa.br.

PMID: 26036184
DOI: 10.1016/j.fsigen.2015.03.015

Abstract

The aim of this study was to estimate the diversity of 30 insertion/deletion (INDEL) markers (Investigator(®) DIPplex kit) in a sample of 519 individuals from six Brazilian states and to evaluate their applicability in forensic genetics. All INDEL markers were found to be highly polymorphic in the Brazilian population and were in Hardy-Weinberg equilibrium. To determine their forensic suitability in the Brazilian population, the markers were evaluated for discrimination power, match probability and exclusion power. The combined discrimination power (CDP), combined match power (CMP) and combined power of exclusion (CPE) were higher than 0.999999, 3.4 × 10(-13) and 0.9973, respectively. Further comparison of 29 worldwide populations revealed significant genetic differences between continental populations and a closer relationship between the Brazilian and European populations.

Keywords: Biallelic; Brazilian population; INDEL; Insertion–deletion polymorphisms; Investigator DIPplex(®) kit.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brazil
Forensic Genetics
Genetics, Population*
Humans
INDEL Mutation*
Linkage Disequilibrium
Polymorphism, Single Nucleotide*