Biopolymer nanoparticles designed for polyunsaturated fatty acid vehiculization: Protein-polysaccharide ratio study

Food Chem. 2015 Dec 1:188:543-50. doi: 10.1016/j.foodchem.2015.05.043. Epub 2015 May 12.

Abstract

Information about the design of biopolymer nanoparticles (BNPs) for polyunsaturated fatty acid (PUFA) vehiculization is provided. Linoleic acid (LA) was used as a model PUFA. The binding ability of LA to β-lactoglobulin (BLG) was applied for obtaining BLG-LA complexes. BLG-LA complex formation was monitored by fluorimetry and it was observed that a moderate heat treatment (60 °C, 10 min) enhanced BLG-LA complexation. Obtaining BNPs involved the electrostatic deposition of high methoxyl pectin (HMP) onto the BLG-LA complex surface. The phase behavior of biopolymer systems was discussed at different Prot:HMP ratio (RProt:HMP, wt.%) levels (1:1-6:1). Absorbance at 600 nm, particle size, and ζ potential were analyzed at pH 4.0. At 1:1-2:1 RProt:HMP, BNPs showed appreciable turbidity, a nanometric diameter (337-364 nm), and a negative ζ potential. Finally, intrinsic and extrinsic fluorimetry was used for examining the HMP protective role at the LA binding site. At 2:1 RProt:HMP, HMP cover could promote significant LA protection in BNPs.

Keywords: Binding properties; Biopolymer nanoparticles; High methoxyl pectin; Linoleic acid; Vehiculization; β-Lactoglobulin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biopolymers / chemistry*
  • Fatty Acids, Unsaturated / chemistry*
  • Lactoglobulins / chemistry
  • Nanoparticles / chemistry*
  • Pectins / chemistry*
  • Polysaccharides / chemistry*

Substances

  • Biopolymers
  • Fatty Acids, Unsaturated
  • Lactoglobulins
  • Polysaccharides
  • Pectins