A novel murine model for evaluating bovine papillomavirus prophylactics/therapeutics for equine sarcoid-like tumours

J Gen Virol. 2015 Sep;96(9):2764-2768. doi: 10.1099/vir.0.000212. Epub 2015 Jun 4.

Abstract

Equine sarcoids are highly recurrent bovine papillomavirus (BPV)-induced fibroblastic neoplasms that are the most common skin tumours in horses. In order to facilitate the study of potential equine sarcoid prophylactics or therapeutics, which can be a slow and costly process in equines, a murine model for BPV-1 protein-expressing equine sarcoid-like tumours was developed in mice through stable transfection of BPV-1 E5 and E6 in a murine fibroblast tumour cell line (K-BALB). Like equine sarcoids, these murine tumour cells (BPV-KB) were of fibroblast origin, were tumorigenic and expressed BPV-1 proteins. As an initial investigation of the preclinical potential of this tumour model for equine sarcoids prophylactics, mice were immunized with BPV-1 E5E6 Venezuelan equine encephalitis virus replicon particles, prior to BPV-KB challenge, which resulted in an increased tumour-free period compared with controls, indicating that the BPV-KB murine model may be a valuable preclinical alternative to equine clinical trials.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bovine papillomavirus 1 / genetics
  • Bovine papillomavirus 1 / physiology*
  • Disease Models, Animal*
  • Horse Diseases / prevention & control
  • Horse Diseases / therapy
  • Horse Diseases / virology*
  • Horses*
  • Mice*
  • Papillomavirus Infections / prevention & control
  • Papillomavirus Infections / therapy
  • Papillomavirus Infections / veterinary*
  • Papillomavirus Infections / virology
  • Skin Neoplasms / prevention & control
  • Skin Neoplasms / therapy
  • Skin Neoplasms / veterinary*
  • Skin Neoplasms / virology