Biodiversity and degradation potential of oil-degrading bacteria isolated from deep-sea sediments of South Mid-Atlantic Ridge

Xiangxing Gao; Wei Gao; Zhisong Cui; Bin Han; Peihua Yang; Chengjun Sun; Li Zheng

doi:10.1016/j.marpolbul.2015.05.065

Biodiversity and degradation potential of oil-degrading bacteria isolated from deep-sea sediments of South Mid-Atlantic Ridge

Mar Pollut Bull. 2015 Aug 15;97(1-2):373-380. doi: 10.1016/j.marpolbul.2015.05.065. Epub 2015 Jun 12.

Authors

Xiangxing Gao¹, Wei Gao², Zhisong Cui¹, Bin Han¹, Peihua Yang³, Chengjun Sun¹, Li Zheng⁴

Affiliations

¹ Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration of China, Qingdao, China.
² Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration of China, Qingdao, China; College of Marine life, Ocean University of China, Qingdao, China.
³ College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
⁴ Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration of China, Qingdao, China. Electronic address: Zhengli@fio.org.cn.

PMID: 26077158
DOI: 10.1016/j.marpolbul.2015.05.065

Abstract

The indigenous oil-degrading bacterial consortia MARA and MARB were enriched from the deep-sea sediments of South Mid-Atlantic Ridge (MAR) with crude oil as sole carbon and energy sources. Biodiversity and community analyses showed that members of α-Proteobacteria were the key players in consortium MARA, whereas those of γ-Proteobacteria were the key players in consortium MARB, which were studied by MiSeq sequencing method. Gravimetric method estimated the oil degradation rates of MARA and MARB to be 63.4% and 85.8%, respectively, after 20d. Eleven cultivable oil degraders with different morphologies were isolated. These strains were identified as Alcanivorax, Bacillus, Dietzia, Erythrobacter, Marinobacter, Nitratireductor, and Oceanicola based on 16S rRNA gene sequences. Three strains belonging to Dietzia exhibited the highest oil degradation capability. Results indicated that the intrinsic biodegradation capacity of oil contaminants by indigenous microbial communities exists in South MAR sediments.

Keywords: Crude oil biodegradation; Metagenome; Microbial consortium; Mid-Atlantic Ridge; Sediments.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Atlantic Ocean
Bacteria / genetics*
Bacteria / metabolism
Biodegradation, Environmental
Biodiversity*
Geologic Sediments / microbiology
Microbial Consortia / genetics
Microbial Consortia / physiology*
Petroleum / metabolism*
Phylogeny
RNA, Ribosomal, 16S / genetics
Water Microbiology

Substances

Petroleum
RNA, Ribosomal, 16S