The receiver operating characteristic (ROC) curve analysis has been widely used in the evaluation of screening validity in Epidemiology and in determining the optimal operating point. At present, theories about ROC curves and computation methods have been established mainly based on cross-sectional data. It is important to understand the characteristics and the progress of ROC application in order to explore more researches on optimal cut-off value, which could also give suggestions on non-communicable diseases (NCD) prevention and control. This study reviewed the application of ROC curve analysis and softwares in NCD and pointed out the future work.