Oxymatrine ameliorates renal ischemia-reperfusion injury from oxidative stress through Nrf2/HO-1 pathway

Acta Cir Bras. 2015 Jun;30(6):422-9. doi: 10.1590/S0102-865020150060000008.

Abstract

Purpose: To investigate if oxymatrine pretreatment could ameliorate renal I/R injury induced in rats and explore the possible role of oxymatrine in Nrf2/HO-1 pathway.

Methods: Unilaterally nephrectomized rats were insulted by I/R in their left kidney. Twenty four rats were randomly divided into three groups: sham group, I/R + saline-treated group, I/R + OMT-treated group. Oxymatrine or vehicle solution was administered intraperitoneally injected 60 min before renal ischemia, respectively. Renal function, histology, makers of oxidative stress, cell apoptosis and Nrf2/HO-1 expressions were assessed.

Results: Oxymatrine pretreatment exhibited an improved renal functional recovery, alleviated histological injury and oxidative stress, inhibiting tubular apoptosis, and accompanied by upregulated the expression of Nrf2/HO-1 proteins.

Conclusion: Oxymatrine may attenuate renal ischemia/reperfusion injury, and this renoprotective effect may be through activating the Nrf2/HO-1 pathway.

Publication types

  • Evaluation Study

MeSH terms

  • Alkaloids / pharmacology*
  • Alkaloids / therapeutic use
  • Animals
  • Antioxidants / pharmacology*
  • Antioxidants / therapeutic use
  • Apoptosis / drug effects
  • Blotting, Western
  • Disease Models, Animal
  • Heme Oxygenase-1 / analysis
  • Heme Oxygenase-1 / metabolism*
  • Immunohistochemistry
  • In Situ Nick-End Labeling
  • Kidney / blood supply*
  • Kidney / pathology
  • Male
  • NF-E2-Related Factor 2 / analysis
  • NF-E2-Related Factor 2 / metabolism*
  • Oxidative Stress / drug effects*
  • Quinolizines / pharmacology*
  • Quinolizines / therapeutic use
  • Random Allocation
  • Rats, Sprague-Dawley
  • Reperfusion Injury / metabolism
  • Reperfusion Injury / pathology
  • Reperfusion Injury / prevention & control*
  • Reproducibility of Results
  • Time Factors
  • Treatment Outcome

Substances

  • Alkaloids
  • Antioxidants
  • NF-E2-Related Factor 2
  • Quinolizines
  • oxymatrine
  • Heme Oxygenase-1