New Metrics for Evaluating Viral Respiratory Pathogenesis

PLoS One. 2015 Jun 26;10(6):e0131451. doi: 10.1371/journal.pone.0131451. eCollection 2015.

Abstract

Viral pathogenesis studies in mice have relied on markers of severe systemic disease, rather than clinically relevant measures, to evaluate respiratory virus infection; thus confounding connections to human disease. Here, whole-body plethysmography was used to directly measure changes in pulmonary function during two respiratory viral infections. This methodology closely tracked with traditional pathogenesis metrics, distinguished both virus- and dose-specific responses, and identified long-term respiratory changes following both SARS-CoV and Influenza A Virus infection. Together, the work highlights the utility of examining respiratory function following infection in order to fully understand viral pathogenesis.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Chlorocebus aethiops
  • Disease Models, Animal
  • Dogs
  • Influenza A Virus, H1N1 Subtype*
  • Lung / pathology
  • Lung / virology
  • Madin Darby Canine Kidney Cells
  • Mice
  • Mice, Inbred C57BL
  • Orthomyxoviridae Infections / physiopathology*
  • Plethysmography, Whole Body*
  • Predictive Value of Tests
  • Severe Acute Respiratory Syndrome / physiopathology*
  • Severe acute respiratory syndrome-related coronavirus*
  • Vero Cells
  • Viral Load
  • Virus Replication / physiology