Quantification of pollen tube attraction in response to guidance by female gametophyte tissue using artificial microscale pathway

Abstract

We developed two types of artificial platforms, T-junction and crossroad microchannel devices, and obtained guidance response ratio of pollen tubes to the female tissue as 56-57%. The crossroad device was also able to collect the attracted pollen tubes with high purity, which is useful for future omics analysis.

Keywords: Micro-electro-mechanical system; Microchannel; Microchip; Ovule; Plant reproduction; Pollen tube; Pollen tube guidance; Polydimethylsiloxane; Purification; Torenia fournieri.