Abstract
A comparative analysis of ethanol extracts from peel, pulp and seed of Prunus persica var. platycarpa (Tabacchiera peach) was done. The total phenol, flavonoid and carotenoid content as well as the antioxidant properties by using different in vitro assays (DPPH, ABTS, FRAP, Fe-chelating, β-carotene bleaching test) were evaluated. Pulp extract was subjected to liquid chromatography-electrospray-tandem mass spectrometry (HPLC-ESI-MS/MS). Gallic acid, protocatechuic acid, protocatechualdehyde, chlorogenic acid, p-coumaric acid, and ferulic acid were identified as main constituents. Pulp extract was characterized by the highest total phytonutrients content and exhibited the highest antioxidant activity in all in vitro assays (IC(50) values of 2.2 μg/mL after 60 min of incubation by using β-carotene bleaching test and 2.9 μg/mL by using Fe-chelating assay). Overall, the obtained results suggest that P. persica var. platycarpa displays a good antioxidant activity and its consumption could be promoted.
MeSH terms
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Antioxidants / analysis
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Antioxidants / pharmacology*
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Benzaldehydes / analysis
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Benzaldehydes / pharmacology
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Benzothiazoles / metabolism
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Biphenyl Compounds / metabolism
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Catechols / analysis
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Catechols / pharmacology
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Chlorogenic Acid / analysis
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Chlorogenic Acid / pharmacology
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Chromatography, High Pressure Liquid
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Coumaric Acids / analysis
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Coumaric Acids / pharmacology
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Fruit / chemistry*
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Gallic Acid / analysis
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Gallic Acid / pharmacology
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Humans
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Hydroxybenzoates / analysis
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Hydroxybenzoates / pharmacology
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Oxidation-Reduction
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Picrates / metabolism
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Plant Extracts / chemistry
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Plant Extracts / pharmacology*
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Propionates
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Prunus persica / chemistry*
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Seeds / chemistry
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Species Specificity
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Sulfonic Acids / metabolism
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Tandem Mass Spectrometry
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beta Carotene / metabolism
Substances
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Antioxidants
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Benzaldehydes
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Benzothiazoles
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Biphenyl Compounds
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Catechols
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Coumaric Acids
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Hydroxybenzoates
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Picrates
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Plant Extracts
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Propionates
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Sulfonic Acids
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beta Carotene
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2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
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Chlorogenic Acid
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protocatechuic acid
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protocatechualdehyde
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Gallic Acid
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ferulic acid
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1,1-diphenyl-2-picrylhydrazyl
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p-coumaric acid