Objective: To construct a lentiviral vector carrying tumor necrosis factor superfamily member 14 (TNFSF14) gene, infect tongue cancer Tca8113 cells in vitro, and observe the effect on infected Tca8113 cells.
Methods: A lentiviral vector containing TNFSF14 gene was constructed and used to infect the Tca8113 cells. After selected by puromycin, the level of TNFSF14 mRNA in Tca8113 cells was detected by real-time quantitative PCR. Cell proliferation activity and cell circle were determined respectively by MTT assay and flow cytometry (FCM). And the cell migration ability was measured by Transwell(TM) assay.
Results: Compared with the control group, the expression of TNFSF14 mRNA increased in the infected cells. MTT assay and FCM showed TNFSF14 promoted the proliferation of Tca8113 cells. Transwell™ assay showed TNFSF14 boosted the migration ability of Tca8113 cells.
Conclusion: The proliferation and migration would be enhanced in Tca8113 cells with over-expressed TNFSF14.