Abstract
One new β-carboline alkaloid 7-methoxy-(9H-β-carbolin-1-il)-(E)-1-propenoic acid (1) together with 9-methoxycanthin-6-one (2) and 9-hydroxycanthin-6-one (3) were isolated from the hairy-root cultures of Eurycoma longifolia. The effects of these compounds on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 cells were investigated. Compound 1 strongly inhibited the production of NO while 2 and 3 having weak or inactive effect. Consistently, compound 1 decreased the expression of cyclooxygenase-2 and inducible nitric oxide synthase.
Keywords:
Eurycoma longifolia; hairy-root cultures; β-carboline alkaloid.
MeSH terms
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Alkaloids / isolation & purification
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Alkaloids / pharmacology*
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / chemistry
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Carbolines / isolation & purification
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Carbolines / pharmacology*
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Cyclooxygenase 2 Inhibitors / pharmacology
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Drug Evaluation, Preclinical / methods
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Eurycoma / chemistry*
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Eurycoma / cytology
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Indole Alkaloids / isolation & purification
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Indole Alkaloids / pharmacology
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Macrophages / metabolism
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Mice
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Nitric Oxide / metabolism
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Nitric Oxide Synthase Type II / antagonists & inhibitors
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Nitric Oxide Synthase Type II / metabolism
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Plant Roots / chemistry
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Plant Roots / cytology
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Plants, Medicinal / chemistry
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Plants, Medicinal / cytology
Substances
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7-methoxy-(9H-beta-carbolin-1-il)-(E)-1-propenoic acid
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9-hydroxycanthin-6-one
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9-methoxycanthin-6-one
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Alkaloids
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Anti-Inflammatory Agents, Non-Steroidal
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Carbolines
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Cyclooxygenase 2 Inhibitors
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Indole Alkaloids
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Lipopolysaccharides
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Nitric Oxide
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Nitric Oxide Synthase Type II
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Nos2 protein, mouse