Identification of ribonucleotide reductase mutation causing temperature-sensitivity of herpes simplex virus isolates from whitlow by deep sequencing

Tohru Daikoku; Yukari Oyama; Misako Yajima; Tsuyoshi Sekizuka; Makoto Kuroda; Yuka Shimada; Kazuhiko Takehara; Naoko Miwa; Tomoko Okuda; Tetsutaro Sata; Kimiyasu Shiraki

doi:10.1002/ccr3.270

Identification of ribonucleotide reductase mutation causing temperature-sensitivity of herpes simplex virus isolates from whitlow by deep sequencing

Clin Case Rep. 2015 Jun;3(6):461-7. doi: 10.1002/ccr3.270. Epub 2015 Apr 22.

Authors

Affiliations

¹ Department of Virology, University of Toyama Toyama, 930-0194, Japan.
² Pathogen Genomics Center, National Institute of Infectious Diseases 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan.
³ Department of Dermatology, Kanazawa University Graduate School of Medical Science Kanazawa, Ishikawa, 920-8640, Japan.
⁴ Department of Gynecology and Obstetrics, University of Toyama Toyama, 930-0194, Japan ; Department of Obstetrics and Gynecology, Shakaihoken Takaoka Hospital Takaoka, Toyama, 933-0115, Japan.
⁵ Department of Virology, Toyama Institute of Health Toyama, 939-0363, Japan.

Abstract

Herpes simplex virus 2 caused a genital ulcer, and a secondary herpetic whitlow appeared during acyclovir therapy. The secondary and recurrent whitlow isolates were acyclovir-resistant and temperature-sensitive in contrast to a genital isolate. We identified the ribonucleotide reductase mutation responsible for temperature-sensitivity by deep-sequencing analysis.

Keywords: Herpes simplex virus; high-throughput DNA sequencing; reactivation; ribonucleotide reductase; temperature-sensitive (Ts); thymidine kinase deficient.

Publication types

Case Reports