Practical Radiation Damage-Induced Phasing

Methods Mol Biol. 2016:1320:205-18. doi: 10.1007/978-1-4939-2763-0_13.

Abstract

Although crystallographers typically seek to mitigate radiation damage in macromolecular crystals, in some cases, radiation damage to specific atoms can be used to determine phases de novo. This process is called radiation damage-induced phasing or "RIP." Here, we provide a general overview of the method and a practical set of data collection and processing strategies for phasing macromolecular structures using RIP.

MeSH terms

  • Crystallization
  • Crystallography, X-Ray / methods*
  • Data Collection
  • Macromolecular Substances
  • Models, Molecular
  • Molecular Structure
  • Nucleic Acids / chemistry*
  • Nucleic Acids / radiation effects
  • Radiation
  • Reproducibility of Results
  • Software
  • Spectrophotometry
  • Synchrotrons

Substances

  • Macromolecular Substances
  • Nucleic Acids