Iterative phasing for fluctuation X-ray scattering

Proc Natl Acad Sci U S A. 2015 Aug 18;112(33):10286-91. doi: 10.1073/pnas.1513738112. Epub 2015 Aug 3.

Abstract

Fluctuation X-ray scattering (FXS) is an extension of small- and wide-angle X-ray scattering in which the X-ray snapshots are taken below rotational diffusion times. This technique, performed using a free electron laser or ultrabright synchrotron source, provides significantly more experimental information compared with traditional solution scattering methods. We develop a multitiered iterative phasing algorithm to determine the underlying structure of the scattering object from FXS data.

Keywords: fluctuation scattering; iterative phasing; polar Fourier transform.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Algorithms
  • Crystallization
  • Fourier Analysis
  • Image Processing, Computer-Assisted / methods*
  • Imaging, Three-Dimensional
  • Lasers
  • Molecular Structure
  • Scattering, Radiation*
  • Scattering, Small Angle
  • Synchrotrons
  • X-Rays*