Performance of the CLSI Carba NP and the Rosco Carb Screen Assays Using North American Carbapenemase-Producing Enterobacteriaceae and Pseudomonas aeruginosa Isolates

J Clin Microbiol. 2015 Oct;53(10):3370-3. doi: 10.1128/JCM.01547-15. Epub 2015 Aug 12.

Abstract

This study compared the performance of the Carba NP assay, published by the Clinical and Laboratory Standards Institute, and the Rosco Rapid Carb Screen kit. Carba NP had superior sensitivity, but both assays required an increased inoculum to detect carbapenemase production in isolates with blaNDM, blaIMP, and blaOXA-48.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / analysis*
  • Enterobacteriaceae / enzymology*
  • Gram-Negative Bacterial Infections / microbiology
  • Humans
  • Microbiological Techniques / methods*
  • North America
  • Pseudomonas aeruginosa / enzymology*
  • Sensitivity and Specificity
  • beta-Lactamases / analysis*

Substances

  • Bacterial Proteins
  • beta-Lactamases
  • carbapenemase