Orthogonal bioorthogonal chemistries

David M Patterson; Jennifer A Prescher

doi:10.1016/j.cbpa.2015.07.006

Orthogonal bioorthogonal chemistries

Curr Opin Chem Biol. 2015 Oct:28:141-9. doi: 10.1016/j.cbpa.2015.07.006. Epub 2015 Aug 10.

Authors

David M Patterson¹, Jennifer A Prescher²

Affiliations

¹ Department of Chemistry, University of California, Irvine, CA 92697, USA.
² Department of Chemistry, University of California, Irvine, CA 92697, USA; Department of Molecular Biology & Biochemistry, University of California, Irvine, CA 92697, USA; Department of Pharmaceutical Sciences, University of California, Irvine, CA 92697, USA. Electronic address: jpresche@uci.edu.

PMID: 26276062
DOI: 10.1016/j.cbpa.2015.07.006

Abstract

Bioorthogonal reactions have long been used to examine individual biomolecules in living systems. Studies of multi-component networks demand not only reliable bioorthogonal chemistries, but also combinations of reactions that can be used in tandem. Such 'orthogonal bioorthogonal' transformations have been reported in recent years, and these chemistries are enabling new explorations into biology. This article highlights the development of orthogonal bioorthogonal reactions and their application in multi-target imaging and macromolecule assembly. Methods to tune and control orthogonal reactivity are also discussed, along with prospects for identifying new classes of compatible reactions.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Alkynes / chemistry
Animals
Azides / chemistry
Biochemistry / methods*
Humans
Models, Molecular
Molecular Imaging / methods*
Proteins / analysis*
Proteins / metabolism

Substances

Alkynes
Azides
Proteins