miR-211 promotes non-small cell lung cancer proliferation by targeting SRCIN1

Tumour Biol. 2016 Jan;37(1):1151-7. doi: 10.1007/s13277-015-3835-y. Epub 2015 Aug 16.

Abstract

MicroRNAs (miRNAs) are a class of small non-coding RNAs that, when dysregulated, are involved in the initiation and progression of various cancers, including lung cancer, in humans. In the current study, qRT-PCR was performed to measure miR-211 expression in human non-small cell lung cancer (NSCLC) cell lines and tissues. Cell proliferation, cell cycle, colony formation, and invasion were performed to detect the functional role of miR-211 in human NSCLC cell line. We used luciferase reporter assay to find the potential target of miR-211. We found that miR-211 expression was upregulated in human non-small cell lung cancer (NSCLC) cell lines and tissues. The overexpression of miR-211 enhanced NSCLC cell proliferation, colony formation, and invasion. SRC kinase signaling inhibitor 1 (SRCIN1) was identified as a direct target of miR-211. SRCIN1 silencing promoted cell proliferation, and SRCIN1 expression was downregulated in human NSCLC cell lines. Thus, miR-211 may function as an oncogenic miRNA in NSCLC, partly by regulating SRCIN1, and the modulation of miR-211 expression represents a potential strategy for the treatment of NSCLC patients.

Keywords: MiR-211; MicroRNA; Non-small cell lung cancer; SRCIN1.

MeSH terms

  • A549 Cells
  • Adaptor Proteins, Vesicular Transport / metabolism*
  • Carcinoma, Non-Small-Cell Lung / metabolism*
  • Cell Line, Tumor
  • Cell Proliferation
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic*
  • Genes, Reporter
  • Humans
  • Lung Neoplasms / metabolism*
  • MicroRNAs / metabolism*
  • Neoplasm Invasiveness

Substances

  • Adaptor Proteins, Vesicular Transport
  • MIRN211 microRNA, human
  • MicroRNAs
  • SRCIN1 protein, human