Efficient fluorescence energy transfer system between fluorescein isothiocyanate and CdTe quantum dots for the detection of silver ions

Luminescence. 2016 Mar;31(2):356-363. doi: 10.1002/bio.2967. Epub 2015 Aug 17.

Abstract

We report a fluorescence resonance energy transfer (FRET) system in which the fluorescent donor is fluorescein isothiocyanate (FITC) dye and the fluorescent acceptor is CdTe quantum dot (QDs). Based on FRET quenching theory, we designed a method to detect the concentration of silver ions (Ag(+)). The results revealed a good linear trend over Ag(+) concentrations in the range 0.01-8.96 nmol/L, a range that was larger than with other methods; the quenching coefficient is 0.442. The FRET mechanism and physical mechanisms responsible for dynamic quenching are also discussed.

Keywords: energy-transfer efficiency; fluorescence resonance energy transfer; fluorescence spectrum; quantum dots; quenching method.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cadmium Compounds / chemistry*
  • Fluorescence Resonance Energy Transfer*
  • Fluorescent Dyes / chemistry*
  • Ions / analysis
  • Isothiocyanates / chemistry*
  • Quantum Dots*
  • Silver / analysis*
  • Tellurium / chemistry*

Substances

  • Cadmium Compounds
  • Fluorescent Dyes
  • Ions
  • Isothiocyanates
  • Silver
  • Tellurium
  • cadmium telluride