Abstract
It is well known that plasmid DNA transfection, prior to virus infection, negatively affects infection efficiency. Here, we show that cytosolic plasmid DNA activates the cGAS/STING signaling pathway, which ultimately leads to the induction of an antiviral state of the cells. Using a transient one-plasmid clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system, we generated cGAS/STING-knockout cells and show that these cells can be infected after plasmid DNA transfection as efficiently as nontransfected cells.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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CRISPR-Cas Systems
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Coxsackievirus Infections / metabolism*
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Cytosol / metabolism*
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Flow Cytometry
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Gene Knockout Techniques
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Gene Transfer Techniques*
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Green Fluorescent Proteins
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HeLa Cells
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Humans
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Luminescent Proteins
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Mengovirus / metabolism
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Nucleotidyltransferases / genetics
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Nucleotidyltransferases / metabolism*
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Plasmids / genetics
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Plasmids / metabolism*
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Red Fluorescent Protein
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Signal Transduction / genetics
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Signal Transduction / physiology*
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Transfection / methods
Substances
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Luminescent Proteins
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Membrane Proteins
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STING1 protein, human
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enhanced green fluorescent protein
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Green Fluorescent Proteins
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Nucleotidyltransferases
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cGAS protein, human