Early weaning influences short-term synaptic plasticity in the medial prefrontal-anterior basolateral amygdala pathway

Abstract

Early weaning in rodents reportedly influences behavioral and emotional traits and triggers precocious myelin formation in the anterior basolateral amygdala (aBLA; Ono et al., 2008), where prefrontal efferents terminate. We studied the correlation between behavior and the synaptic properties of the prefrontal-aBLA pathway. Open-field behaviors of adult male rats weaned at either 16 days or 30 days were measured on two consecutive days. On the first day, the rats received a slight footshock that was reportedly insufficient for fear conditioning. Electrophysiological recordings in the prefrontal-aBLA were then performed under urethane anesthesia. Without group differences in the stimulus intensity or the first evoked response, the overall paired-pulse facilitation was significantly lower in the early-weaned group from 25 to 100 ms. At the 25-ms interval, regression values between paired-pulse facilitation and locomotion on the second day were positive/insignificant and negative/significant in early- and control-weaned groups, respectively, and were statistically different between the groups.

Keywords: Early weaning; Electric stimulation; Locomotion; Maternal environment; Open-field; Paired-pulse facilitation; Rearing.