Epstein-Barr Viruses (EBVs) Deficient in EBV-Encoded RNAs Have Higher Levels of Latent Membrane Protein 2 RNA Expression in Lymphoblastoid Cell Lines and Efficiently Establish Persistent Infections in Humanized Mice

J Virol. 2015 Nov;89(22):11711-4. doi: 10.1128/JVI.01873-15. Epub 2015 Sep 2.

Abstract

Functions of Epstein-Barr virus (EBV)-encoded RNAs (EBERs) were tested in lymphoblastoid cell lines containing EBER mutants of EBV. Binding of EBER1 to ribosomal protein L22 (RPL22) was confirmed. Deletion of EBER1 or EBER2 correlated with increased levels of cytoplasmic EBV LMP2 RNA and with small effects on specific cellular microRNA (miRNA) levels, but protein levels of LMP1 and LMP2A were not affected. Wild-type EBV and EBER deletion EBV had approximately equal abilities to infect immunodeficient mice reconstituted with a human hematopoietic system.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cisplatin / pharmacology
  • Herpesvirus 4, Human / genetics*
  • Humans
  • Mice
  • Mice, Knockout
  • Mice, SCID
  • MicroRNAs / genetics
  • RNA, Viral / biosynthesis
  • RNA, Viral / genetics*
  • RNA-Binding Proteins / metabolism
  • Ribosomal Proteins / metabolism
  • Tumor Suppressor Protein p53 / metabolism
  • Viral Matrix Proteins / genetics
  • Viral Matrix Proteins / metabolism*

Substances

  • EBV-associated membrane antigen, Epstein-Barr virus
  • Epstein-Barr virus encoded RNA 1
  • Epstein-Barr virus encoded RNA 2
  • MicroRNAs
  • RNA, Viral
  • RNA-Binding Proteins
  • Ribosomal Proteins
  • Tumor Suppressor Protein p53
  • Viral Matrix Proteins
  • RPL22 protein, human
  • Cisplatin